Thov ua koj tus kheej xaiv raws li qhov xwm txheej ntawm koj lub chaw kuaj mob.
Nws yog ib qho tseem ceeb kom nrhiav tau txoj hauv kev zoo los ntxuav cov qauv vials
Tam sim no, muaj ntau cov qauv khoom ua liaj ua teb (lwm cov khoom siv tshuaj, organic acids, thiab lwm yam) uas yuav tsum tau kuaj los ntawm cov kua chromatography thiab roj chromatography txhua xyoo.Vim muaj ntau tus qauv, muaj ntau tus qauv vials uas yuav tsum tau muab ntxuav thaum lub sij hawm kuaj xyuas, uas tsis tsuas yog nkim sij hawm thiab txo kev ua hauj lwm efficiency, tab sis kuj qee zaum ua rau deviations nyob rau hauv qhov kev sim tau tshwm sim vim kev ua kom huv si. cov qauv huv huv vials.
Cov qauv chromatographic vials feem ntau yog ua los ntawm iav, tsis tshua muaj yog ua los ntawm yas.Cov qauv pov tseg vials yog kim, khib nyiab, thiab ua paug rau ib puag ncig.Ntau lub chaw soj nstuam ntxuav cov qauv vials thiab rov siv dua.
Tam sim no, feem ntau siv cov kev sim tshuaj ntxuav vials yog tsuas yog ntxiv cov tshuaj ntxuav tes, tshuaj ntxuav tes, cov kuab tshuaj organic thiab kua qaub ntxuav, thiab tom qab ntawd kho txhuam cov raj me me.
Txoj kev siv tshuaj txhuam hniav no muaj ntau yam tsis zoo:
Kev siv cov tshuaj detergent haus dej ntau, lub sij hawm ntxuav yog ntev, thiab muaj cov fab tsis yooj yim los ntxuav.Yog tias nws yog cov qauv yas vials, nws yog ib qho yooj yim kom muaj cov cim txhuam hauv phab ntsa vials, uas yuav siv sij hawm ntau ntawm kev ua haujlwm.Rau cov iav iav uas hnyav heev los ntawm lipid thiab protein residues, alkaline lysis tov yog siv los ntxuav, thiab ua tiav cov txiaj ntsig zoo.
Thaum txheeb xyuas cov qauv los ntawm LC / MS / MS, kev ntxuav ntawm cov vials yog qhov tseem ceeb heev.Raws li txoj kev ntxuav ntawm iav, txoj kev ntxuav yog xaiv raws li qib ntawm cov pa phem.Tsis muaj hom kev ruaj ntseg.Txheej txheem txheej txheem:
Option Ib:
1. Ncuav cov tshuaj ntsuam xyuas hauv cov vials qhuav
2. Dip tag nrho cov tshuaj ntsuam xyuas hauv 95% cawv, ntxuav nws ob zaug nrog ultrasonic thiab ncuav nws tawm, vim hais tias cov cawv tau yooj yim nkag mus rau 1.5mL vial thiab tuaj yeem ua rau miscible nrog cov kuab tshuaj organic feem ntau kom ua tiav cov nyhuv ntxuav.
3. Ncuav rau hauv dej huv, thiab ultrasonically ntxuav ob zaug.
4. Ncuav cov tshuaj pleev rau hauv cov vials qhuav thiab ci ntawm 110 degrees Celsius rau 1 mus rau 2 teev.Tsis txhob ci thaum kub kub.
5. Txias thiab txuag.
Kev xaiv thib ob:
1. Yaug nrog dej kais dej ob peb zaug
2. Muab tso rau hauv lub beaker uas muaj dej ntshiab (Millipore pure water machine) thiab sonicate rau 15 feeb
3. Hloov cov dej thiab ultrasound rau 15 feeb
4. Soak hauv lub beaker uas muaj cov ethanol kiag li (Sinopharm Group, Analytical Pure)
5. Thaum kawg, muab nws tawm thiab cia nws cua qhuav.
Kev xaiv peb:
1. Ua ntej soak hauv methanol (chromatographically ntshiab), thiab ultrasonically ntxuav rau 20 feeb, ces ncuav methanol qhuav.
2. Sau cov qauv vials nrog dej, thiab ultrasonically ntxuav rau 20 feeb, ncuav dej.
3. Qhuav cov qauv vials tom qab ntawd.
Xaiv plaub:
Txoj kev ntxuav ntawm cov qauv vials yog tib yam li kev npaj ntawm cov kua theem, thiab lwm yam. Ua ntej, siv cov cawv kho mob kom ntub dej rau ntau tshaj 4 teev, tom qab ntawd ultrasound rau ib nrab ib teev, tom qab ntawd ncuav tawm cov cawv kho mob, thiab siv dej. rau ib nrab ntawm ultrasound.Sij hawm, yaug nrog dej thiab qhuav nws.
Kev xaiv tsib:
Ua ntej, tsau rau hauv cov tshuaj oxidizing muaj zog (potassium dichromate) rau 24 teev, thiab tom qab ntawd siv cov dej deionized hauv ultrasonic Ntxuav peb zaug hauv cov xwm txheej, thiab thaum kawg ntxuav nws nrog methanol ib zaug, thiab tom qab ntawd qhuav nws rau kev siv.
Lub kaus mom septas yuav tsum tau hloov, tshwj xeeb tshaj yog thaum txheeb xyuas cov tshuaj tua kab, txwv tsis pub nws yuav cuam tshuam cov txiaj ntsig ntau.
Tab sis yog tias muaj kev tso cai, sim siv cov khoom siv pov tseg, xws li cov khoom pov tseg PTFE lossis cov khoom siv yas hauv tsev (kwv yees li 0.1 yuan / thooj), thiab cov qauv vials zoo.Rov siv dua thiab tsis tas yuav ntxuav.
Option six:
(1) kev tu kom nyuaj nrog cov txiaj ntsig tau zoo:
Tsis muaj 1.Tom qab siv cov qauv vials, yaug cov qauv vials nrog dej ua ntej, thiab yaug tawm cov qauv seem (koj tuaj yeem co nws ntawm tes tib lub sijhawm);
No 2, ces muab cov qauv vials tso rau hauv cov poov tshuaj dichromate ntxuav cov npuas dej, thiab thaum nws accumulates Thaum koj ncav cuag ib tug npaum li cas los yog thaum koj nyob rau hauv ib tug zoo mus ob peb vas, coj nws tawm ntawm lub lotion tank thiab muab tso rau hauv ib tug yas sieve rau chav ua noj. siv.Yaug kom huv si nrog dej kais.Koj tuaj yeem rov ua dua thiab co hauv nruab nrab;
Tsis muaj 3.Siv cov kais dej los ntxuav ultrasonically 3 zaug tom qab yaug.Nyob ib ncig ntawm, nws yog qhov zoo tshaj rau co tawm cov dej hauv cov qauv vials tom qab txhua zaus ntxuav ultrasonic;
No4, ces siv triple distilled dej (los yog purified dej, deionized dej) nrog 1.3 ultrasonic tu peb zaug;
No5, ces siv chromatographic ntshiab methanol ultrasonic tu 2-3 zaug, nws kuj yog qhov zoo tshaj rau
co cov methanol tawm ntawm cov qauv vials tom qab txhua qhov tu;
Tsis muaj 6.Muab cov qauv vials tso rau hauv qhov cub thiab qhuav nws li 80 degrees, thiab nws tuaj yeem siv tau.
(2) cov qauv vials yuav los kos nrog cov xim sib txawv:
Yog tias koj tau pom tias muaj cov xim me me ntawm cov qauv vials, uas tsis yog rau qhov zoo nkauj, tab sis nws siv.Thaum muas, nws yog qhov zoo tshaj los yuav ntau lub vials ntawm cov xim sib txawv.
Piv txwv li: koj lub chaw kuaj mob qhib ob txoj haujlwm A thiab B tib lub sijhawm.Thawj zaug A qhov project siv cov qauv dawb vials, thiab B project siv cov qauv xiav vials.Tom qab qhov kev xeem tiav lawm, nws raug ntxuav raws li cov qauv saum toj no, thiab qhov kev sim thib ob Thaum lub sijhawm, siv cov qauv xiav vials rau A project, dawb qauv vials rau B project, thiab lwm yam, uas tuaj yeem zam qhov teeb meem los ntawm ua paug rau koj txoj haujlwm.
Sau ntawv kawg
1. Ntau tus kws tsim khoom lag luam tau tawm tswv yim: Siv lub cub tawg ntawm 400 degrees kom ci rau ib nrab ib teev, cov khoom organic tau ploj mus;
2. Muab cov qauv vials tso rau hauv lub qhov cub muffle kom qhuav ntawm 300 degrees Celsius.Tus kws ua haujlwm Agilent los ntawm Beijing tau hais tias thaum nws tuaj rau lub qhov cub muffle, qhov kev sim yuav tsis muaj suab nrov tom qab ci hauv qhov cub muffle ntawm 300 degrees rau 6 teev.
kuj………..
Cov dej ntim me me, cov pear-puab flasks rau rotary evaporation, thiab lwm yam iav rau kev tsom xam lossis pretreatment tuaj yeem ntxuav los ntawm kev xa mus rau txoj kev no.
Post lub sij hawm: Feb-25-2022